Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Crystalgen Inc PBS PH7.2 10X 12 X 500ML
PBS (Phosphate Buffered Saline) pH7.2 10X, with Ca, Mg-500mlUltra pure Phosphate buffered isotonic saline. With calcium and magnesium. Sterile filtered. 1X PBS solutions contains 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 2mM KH2PO4.pH: 7.2Storage: 18-26oC
Sigma Aldrich Fine Chemicals Biosciences DIPSO BioXtra pH 4.0 5.5 2100G
DIPSO BioXtra pH 4.0 5.5 2100G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences Iscoves Modified Dulbeccos
ISCOVES Iscoves Modified Dulbeccos
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul10G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences MES hemisodium salt 98 tit100G
SODIUM MES hemisodium salt 98 tit100G
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline1L
PHOSPHATE Phosphate buffered saline1L
Sigma Aldrich Fine Chemicals Biosciences Tyrodes Salts With sodium500ML
SODIUM Tyrodes Salts With sodium500ML
Sigma Aldrich Fine Chemicals Biosciences MES solution suitable for500ML
MES SOLUTIONon suitable for500ML
