Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 415952-27-1 | methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | Broadpharm | MFCD01452616 | 263.337 | C15H21NO3 | 97.000 | COC(=O)c1ccc(CN2CCCC(CO)C2)cc1 | 1g | 112541923
methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | Broadpharm | 415952-27-1 | MFCD01452616 | 263.337 | C15H21NO3 | 97.000 | COC(=O)c1ccc(CN2CCCC(CO)C2)cc1 | 1g | 112541923
Teknova 1M Tris-HCl, pH 7.5 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Seracare Life Sciences Inc 10X TRIS-GLYCINE-SDS BUFFER
Tris-Glycine-SDS (TGS) is a running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. pH=8.3. Conductivity controlled.
Thermo Scientific Production Chemicals and Services TRIS HCL GMP GRADE 10KG
NC3654762 TRIS HCL GMP GRADE 10KG
Teknova 1M Tris-HCl, pH 9.0 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences TRIS 3 HYDROXYPROPYLTRIAZOLY1G
Tris(3-hydroxypropyltriazolylmethyl)amine, 1g
![eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502181707.JPG-250.jpg)
eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g
Medchem Express | Deschloroclozapine | 10mg | 446270896 | HY-42110 | 1977-07-7 | MFCD01730958 | 292.386 | C18H20N4
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool<|a>
S2 Media 1L 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 in 1L Media Bottle, 1/each
S2Media™ 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 is a ready to use stock buffer often used for immunodetection techniques, including Western blot protocols. To prepare a 1x solution, mix one volume of 10x solution with 9 volumes of 10x buffer solution.• Product meets reference methods and performance criteria.• TBS,10x should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions. • Product is 0.1µm filtered. Bottles are RNase, DNase, and protease free.
Alkali Scientific TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
TRIS Amino (Enzyme Grade) buffer is a high-purity solution designed specifically for enzyme-related applications in molecular biology and biochemistry. This 5kg package provides an ample supply of this reagent, which is widely used for preparing enzyme buffers in assays such as enzyme-linked immunosorbent assays (ELISA), protein assays, and PCR. The TRIS Amino buffer maintains stable pH conditions critical for optimal enzyme activity, and its enzyme-grade quality ensures minimal impurities that could interfere with biochemical reactions. This buffer is ideal for use in experiments where precise pH control is required for enzymatic reactions, as well as for preparing buffers for enzyme activity studies and protein purification.